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MutaPrimer

張貼者:2010年9月19日 上午8:29未知的使用者   [ eddie liu 已於 2011年5月10日 下午8:30 更新 ]
MutaPrimer: Free software to design primers for Stratagene's Quikchange site directed mutagenesis kitsMutaPrimer是一個桌面工具來設計引物Stratagene的QuikChange定點突變試劑盒。



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開發商:PREMIER Biosoft International
原廠網址:http://www.premierbiosoft.com/mutagenesis/index.html
更新日期:2011/05/11
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特點

誘變引物設計:MutaPrimer設計突變引物使用Stratagene的QuikChange定點突變試劑盒,使單/多基地的變化,插入或刪除。

定點突變,已成為一個最重要的分子生物學工具。 它是一種技術引進在選定地點突變的DNA分子,通常是圓形質粒分子。 在一般情況下,定點突變規定,野生型基因序列被稱為。

隨著QuikChange Stratagene公司提供的試劑盒,您可以進行修改和表達載體蛋白突變造成的許多應用,包括探索蛋白質結構與功能的關係,研究基因表達,識別抗藥性的變種,和其他藥物發現活動。 有了這些套件,你可以在幾個地點誘變雙鏈DNA寡核苷酸使用單個突變。

當前版本的MutaPrimer設計引物與Stratagene的QuikChange和QuikChange加大碼工具包。

序列信息:你可以啟動引物搜索過程中所裝載的DNA序列或氨基酸序列或直接從Entrez或從您的本地驅動器。當開始的肽序列,您可以使用“系統中的表達“選項將其變為一個非退化的DNA序列。MutaPrimer達到這個密碼子使用頻率的數據表達系統的選擇。 翻譯工具可用於顯示的蛋白質編碼的野生和突變的DNA序列。

突變體的信息:在基因或蛋白質載入利益只需添加突變信息通過指定的突變類型使用容易使用MutaPrimer屏幕。 啟動搜索, 瞧! 你將看到最好的引物為保證試驗成功。

底漆性能:MutaPrimer誘變引物設計的完全符合引物設計指引Stratagene公司公佈其QuikChange定點突變試劑盒。 根據指引,最重要的參數是理想的Tm,並要求長度為側翼地區。

底漆評價:除了遵守Stratagene的指導方針,MutaPrimer檢查所有可能的二級結構的引物,如二聚體,髮夾,重複和運行並顯示它們的圖形。MutaPrimer然後率所有可用的引物,使用指定的引物屬性和二級結構穩定性。然後報告的最佳誘變引物以及候補(50)。評級算法是建立在可靠的統計優化技術來挑選最好的引物對每個實驗。 我們認為,試驗的成功是創新的保證,這個算法建成MutaPrimer。







Features

Mutagenic primer design: MutaPrimer designs mutagenic primers for use with Stratagene's QuikChange site directed mutagenesis kits which enable single/multiple base changes, insertions or deletions.

Site-directed mutagenesis has become one of the most important tools in molecular biology. It is a technique to introduce mutations at selected sites in a DNA molecule, usually a circular plasmid molecule. In general, site-directed mutagenesis requires that the wild-type gene sequence be known.

With QuikChange kits offered by Stratagene, you can carry out vector modifications and express resultant mutant proteins for many applications including exploration of protein structure-function relationships, study of gene expression, identification of drug resistant variants, and other drug discovery activities. With these kits, you can mutagenize several sites in double stranded DNA using a single mutagenic oligonucleotide.

The current version of MutaPrimer designs primers to work with Stratagene's QuikChange and QuikChange XL Kits.

Sequence information: You can start the primer search process by loading either the DNA sequence or the amino acid sequence directly from Entrez or from your local drive. When starting with the peptide sequence, you can use the “Expression System” option to translate it into a non-degenerate DNA sequence. MutaPrimer achieves this by using the codon frequency data for the selected expression system. The translation tool can be used to display the proteins coded by the both wild and mutant DNA sequences.

Mutant Information: After loading the gene or protein of interest simply add the mutation information by specifying the mutation type using easy to use MutaPrimer screens. Launch the search, and voila! You will be presented with the best primers for assured experimental success.

Primer properties: MutaPrimer designs mutagenic primers that fully comply with the primer design guidelines published by Stratagene for their QuikChange site directed mutagenesis kits. According to the guidelines, the most important parameters are desired Tm and required lengths for the flanking regions.

Primer rating: In addition to complying with Stratagene's guidelines, MutaPrimer checks all possible secondary structures of the primers such as dimers, hairpin, repeats and runs and displays them graphically. MutaPrimer then rates all available primers using the specified primer properties, and the secondary structure stabilities. It then reports the best mutagenic primers along with the alternates (up to 50). The rating algorithm is based on a proven statistical optimization technique to pick the very best primers for every experiment. We believe that assay success is assured by this innovative algorithm built into MutaPrimer.