![]() ------------------------------------ 開發商:PREMIER Biosoft International 原廠網址:http://www.premierbiosoft.com/primerdesign/featuresp/oligo_calculator.html ------------------------------------------------------------------------------ 特點 新改進的搜索算法
自動序列檢索快速檢索批直接序列從 GenBank中的方案和dbSNP數據庫使用加入/胃腸號碼和檢測標識。 使用多種檢索設施,序列可以加載本地驅動器。自動避同源性與模板結構特異性引物可以通過執行 BLAST搜索和尋找模板結構,從內部程序。 結果這兩個搜索使用而設計引物。 該地區的同源性,並表現出顯著跨結構的模板自動避免在引物設計。評估預先設計的引物底漆總理分析和評價的屬性預先設計的引物和隊伍他們為你裁定多遠的引物是從他們的預期目標值。 此功能特別有用,如果你正在使用出版或預先設計的很好的證明引物。對於一個特定的引物,引物總理甚至可以設計一個兼容的設置。 例如,對於一個預先設計或發表了底漆,底漆總理可以找到一個兼容的反向引物,以滿足所有的設計標準。 您可以評估一個正/反向引物或引物,甚至無需輸入序列的模板。 底漆總理顯示所有的屬性寡正在分析和出口他們在HTML或CSV文件格式。 引物的排名,讓您判斷他們如何密切配合目標值。 順序查看選定的序列可以被視為在3個或10個鹼基,每塊,單股或雙股。 所設計的引物標記,引物的意義上有藍色和紅色的反義引物。 選定的單核苷酸多態性標記為粉紅色。 的同源性區域突出顯示為黃色和基地參加模板結構強調。 您可以導出“序列的意見”隨著引物設計結果以CSV或HTML格式。生成報告您可以創建演示文稿的質量檢測報告,為您的筆記本電腦設計的實驗室和與同事共享信息。 該報告形象化的立場上的引物序列,包括候補名單的引物,顯示引物,擴增和序列特性和設計參數所使用的引物設計方案。本地及桌面高爐選項您可以炸開序列數據庫對當地習俗和桌面使用本地BLAST的選擇。 創建一個數據庫的序列或FASTA格式文本格式在您的計算機和您的查詢序列高爐從入門總理。 結果,然後用於引物設計。 這種支持是特別有用,如果你不想提交您的數據在互聯網上或者如果您正在使用未公開的信息。計算器一個內置的計算器來計算的GC%,顯示基地數量,使您能夠獲得的互補和反向互補鏈。數據和數據庫管理可以創建多個項目。 多種實驗數據可以很容易地通過創建單獨的項目管理,為每個實驗。 維護一個本地數據庫的序列信息和搜索結果。Features New Improved Search Algorithms
Automatic Sequence RetrievalQuickly retrieves batches of sequences directly into the program from GenBank and dbSNP using accession/GI numbers and assay Ids. Using the multiple retrieval facility, sequences can be loaded from local drives.Automatic Homology & Template Structure AvoidancePrimer specificity can be achieved by performing BLAST search and search for template structures from within the program. The results of both these searches are used while designing primers. The regions that exhibit significant cross homologies and template structures are avoided automatically during primer design.Evaluate Pre-designed PrimersPrimer Premier analyzes and evaluates the properties of pre-designed primers and ranks them for you to adjudge how far the primers are from their desired target values. This feature is especially useful if you are working with published or pre-designed well proven primers.For a particular primer, Primer Premier can even design a compatible set. For example, for a pre-designed or published forward primer, Primer Premier can find a compatible reverse primer that meets all the design criteria. You can evaluate a forward/reverse primer or a primer pair without even having to enter the template sequence. Primer Premier displays all the properties of the oligos being analyzed and exports them in an HTML or csv file format. The primers are ranked enabling you to judge how closely they meet the target values. Sequence ViewThe selected sequence can be viewed in 3 or 10 bases per block, single stranded or double stranded. The designed primers are marked, the sense primer in blue and the antisense primer in red. The selected SNPs are marked in pink. The homologies regions are highlighted in yellow and the bases participating in template structures are underlined. You can export the “sequence view” along with the primer design results in csv or HTML format.Generate ReportYou can create a presentation quality report for the assays you designed for lab notebooks and for sharing information with colleagues. The report visualizes the positions of the primers on the sequence, includes a list of the alternate primers, displays primers, amplicon and sequence properties and the design parameters used by the primer design program.Local & Desktop BLAST OptionsYou can BLAST your sequences against local custom databases using the Local and Desktop BLAST options. Create a database of sequences in text or FASTA format on your computer and BLAST your query sequence from Primer Premier. The results are then used for primer design. This support is especially useful if you do not want to submit your data on the Internet or if you are working with unpublished information.CalculatorA built in calculator calculates the GC%, displays the base count and enables you to obtain the complementary and the reverse complementary strands.Data & Database ManagementMultiple projects can be created. Data of multiple experiments can be easily managed by creating separate project for each experiment. Maintains a local database for sequence information and search results. |