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Gel Pro analysis

張貼者:2011年11月25日 上午1:22未知的使用者   [ service orderble 已於 2012年8月8日 下午2:38 更新 ]
Media CyberneticsGel-Pro Analyzer software increases laboratory productivity and efficiency by eliminating the time consuming steps involved in manually analyzing Southern, Northern, and Western blots, dot and slot blots, and quantitative PCR. Gel-Pro was jointly designed by Media Cybernetics and molecular biologists for intuitive, easy-to-use analysis without compromising the features necessary to achieve meaningful results. 




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開發商: Media Cybernetics, Inc.
更新日期:2011/11/25
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General
  • Non-destructive annotation overlay
  • User defined color assignment
  • 3D surface plot to aid data interpretation
  • Full support for acquisition of sequences and extraction of frames for analysis
  • Undo buffer
  • Recovery of analysis progress from inadvertent computer power loss
  • Support for GLP/CFR 21 Part 11 Compliance via audit trail and image/file signature

Lanes

  • Option for non-uniform lane width; individual lanes may be different widths
  • Active lane in profile is outlined boldly both on the image and the profile which aids the user when many lanes are displayed
Bands
  • Interactive band height sensitivity to aid in proper automatic band finding
  • Define band pattern to achieve better band resolution for closely-spaced, distorted bands
  • Bands within rows may be determined by MW or Rf
  • Band extents may be edited via handles on the profile or on the image overlay
  • User may select position for band labels for optimum clarity
  • Correct for misshapen bands to give better resolution
Lane Profile
  • Bands may be indicated by peak position or center of mass
  • Lane bitmap may be displayed under the profile to aid interpretation
  • Bands may be indicated by brackets, dotted lines or cross-hatching
  • Band indicators are arrows to pinpoint band position
  • X and Z scale zooming for close examination
  • Easy cycling and selection of the lanes via the profile dialog display
Background
  • New rolling disk algorithm
  • Option to display image with background subtracted
  • Interactive feedback of results as the methods and parameters are changed to optimize results
Quantitation/Results
  • When bands, lanes or rows are selected, the corresponding elements on the image are outlined boldly to aid user interpretation
  • Amount may be determined by the area in the profile under the entire profile or only the area within the bands
  • Supports multiple calibration curves for a single image
  • The row band graph shows a bar chart of the amounts within a row across each lane
  • Presence/absence may be indicated matched to lane 1 via 0, 1, -1 assignment for easy typing
Molecular Weight
  • Hyperbolic cosecant curve fitting produces more accurate MW calculation for small DNA fragments (under 1,000 base pairs) run in polyacrylamide
  • Polynomial slant fit lines may be specified to follow uneven migration more closely and give more accurate results
  • Correct for variable well origins
Dot/Blot
  • User may ask to have missing spots filled in automatically for a regular array
    • An area filter tolerance may be specified to sort out actual spots from background contamination.
  • Area Density
    • Free form or auto-trace definition of spots of interest
    • Extensive table of measurements and measurement statistics
  • Colony Counting
    • Full complement of automatic and manual spot finding
    • User may edit, add, split, merge spots
    • Numerous filtering and measurement options
    • Extensive table of measurements and measurement statistics
    • Automatic classification by user criteria
  • Rich Edit Window